Deleted
Sample Discontinued
Experience: None
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Coding

Part:BBa_K606045:Design

Designed by: Cyrille Pauthenier   Group: iGEM11_Paris_Bettencourt   (2011-09-18)


T7 RNA polymerase double amber


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The quick change mutagenesis has been done with a single long primer of 94 bp. So the mutagenesis is quite hard to do.


Source

The part K145001 has been mutated in two tyrosine site using Site Directed Quick Change mutagenesis.

References